Library-free methylation sequencing with bisulfite padlock probes.

Journal: 
Nat Methods
Publication Year: 
2012
Authors: 
D Diep , N Plongthongkum , A Gore , H L Fung , R Shoemaker , K Zhang
Public Summary: 
Scientific Abstract: 
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.

© 2013 California Institute for Regenerative Medicine