Research Training Program II
Research Training Program II
Research Training II
Here we propose a comprehensive doctoral, postdoctoral and clinical researcher training program designed to develop the next generation of researchers in the field of regenerative medicine. This field, which is centered around the comprehensive understanding, use and manipulation of stem and progenitor cells, promises to revolutionize the way that human diseases and disorders are treated. Advancing the goals of CIRM to develop new treatments for human disease based on stem and progenitor cells will likely require the understanding and application of multiple technologies. Researchers in this field will need to understand multiple disciplines and participate in multi-disciplinary research teams where each of the participants understands the capabilities and shortcomings of each other's technologies. Trainees will be recruited from within existing labs and by external recruitment. Our stem cell training program will emphasize broad, cross-disciplinary training, exposing trainees to concepts and techniques in diverse fields such as stem cell biology, biomedical engineering, pre-clinical development and clinical practice. Courses have been tailored to address the needs of the researchers of the future. All incoming trainees will take part in an intense, two week-long stem cell boot camp in which they will learn the ethical conduct of hypothesis driven research, advanced techniques in cell, DNA, RNA and protein analysis and participate in team building exercises in hypothesis driven research. In the second week of the course they will take the stem cell techniques course in which they will learn practical methods of stem cell isolation, culture and analysis. Journal clubs will allow fellows to keep abreast of the latest developments in the field. In addition we will host monthly seminars where experts present the latest developments in multiple fields related to stem cell research and regenerative medicine including developmental biology, bioengineering, molecular biology, etc. In this way fellows can be updated on the latest developments in the field broadly defined. Lectures will be recorded for web access so that topics can be revisited at leisure and accessed by the entire stem cell research community. Quarterly half-day workshops will expose trainees first-hand to patients and disease advocates, in order to discuss the real-world challenges facing treatment. An annual meeting will allow the administration to determine progress of all trainees as well as allowing trainees to share their results with experts in the field and to develop networking skills. The overall goal will be to train researchers capable of carrying out multidisciplinary research and developing new treatments for new human disease.
Statement of Benefit to California:
A primary goal of Proposition 71 is to translate basic stem cell research to clinical applications. The disability and loss of earning power and personal freedom resulting from a disease or disorder are devastating and create a financial burden for California in addition to the suffering caused to patients and their families. Therapies using human embryonic stem cells (hES cells) have the potential to change millions of lives. Using hES cells as models of disease will help us understand the underlying causes of disease and likely aid in the development of drugs to treat those diseases. For the potential of hES cells to be realized, California researchers need the personnel to develop hES cells into viable treatments. Therefore, the raison d’etre for the proposed program is to provide training to the next generation of stem cell researchers capable of advancing the development of new methods of treating human disease. The breadth and depth of the stem cell biology and regenerative medicine research program, which has already made important advances and secured significant funding from CIRM, will act as the core around which all training will be organized. Anticipated benefits of our Training Program to the Citizens of California include: 1. Creation of an Program that will attract the best and brightest minds to the state 2. Development of new cell-based treatments for a variety of diseases and disorders 3. Generation of new techniques for using stem cells (and derived cells) to deliver drugs or other agents to tissues, thereby developing new treatment methods 4. Improved methods for understanding normal development and environmental risks to the early embryo 5. Improved methods for detecting and understanding effects of toxicants in the environment and workplace 6. Improved clinical trial methodology that will directly impact human testing of stem cell therapies 7. Development of new improved methods for developing and testing drugs for treating disease 8. Transfer of new technologies and intellectual property to the public realm with resulting IP revenues coming into the state 9. Creation of new biotechnology spin-off companies based on generated intellectual property 10. Creating interdisciplinary research teams that will have a competitive edge for obtaining funding from out of state 11. Development of researchers and clinicians that will establish clinical research programs in the state. 12. Creation of new jobs in the biotechnology sector. It is anticipated that, in the long term, the return to the State in terms of revenue, health benefits for its Citizens and job creation will be significant.
Year 1The training program funded by the California Institute for Regenerative Medicine continues to be the centerpiece of the stem cell research program. Trainees are involved in research in many schools of the campus including biological sciences, medicine and engineering and are dissecting questions associated with the use of stem cells for regenerative medicine. The availability of such a training program has been of enormous benefit in recruiting new faculty since they see the availability of support for new trainees. This has resulted in the recruitment of new faculty from some of the finest institutions in the United States including Ivy League schools. Therefore, this has greatly benefited research on the campus and within California. Through this training grant funding, the trainees have been able to attend many national stem cell and regenerative medicine meetings which has allowed them to both present their data and to get feedback from experts in the field. The trainees have also participated in many public outreach activities in which they have had the opportunity to present their work to a lay audience. With public funding of research we feel it is a responsibility to describe our work to the people who fund it. Moreover, the ability to describe science to a lay audience for fundraising purposes is becoming an important aspect of scientific training. During the reporting period the trainees also had the opportunity to attend two locally organized meetings, one highlighting research on the campus and another international meeting on stem cells that has become the leading meeting of its’ kind in the world. The students, postdocs and clinical fellows have also published many important papers describing their work which have advanced the field of regenerative medicine.
- PLoS One (2012) Characterizing the radioresponse of pluripotent and multipotent human stem cells. (PubMed: 23272054)
- Integr Biol (Camb) (2012) Advancing practical usage of microtechnology: a study of the functional consequences of dielectrophoresis on neural stem cells. (PubMed: 22892587)
- J Neurosci (2012) BMP4 Sufficiency to Induce Choroid Plexus Epithelial Fate from Embryonic Stem Cell-Derived Neuroepithelial Progenitors. (PubMed: 23136431)
- Lab Chip (2012) Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells. (PubMed: 22460949)
- Cell Stem Cell (2012) Induced Pluripotent Stem Cells from Patients with Huntington's Disease Show CAG-Repeat-Expansion-Associated Phenotypes. (PubMed: 22748968)
- Stem Cells (2011) A Novel Role for an RNA Polymerase III Subunit POLR3G in Regulating Pluripotency in Human Embryonic Stem Cells. (PubMed: 21898682)
- J Vis Exp (2011) Stem cell transplantation strategies for the restoration of cognitive dysfunction caused by cranial radiotherapy. (PubMed: 22042021)
- J Vis Exp (2011) Quantifying cognitive decrements caused by cranial radiotherapy. (PubMed: 22042060)
- J Cardiovasc Transl Res (2011) Biomaterials to prevascularize engineered tissues. (PubMed: 21892744)
- PLoS One (2011) L1TD1 Is a Marker for Undifferentiated Human Embryonic Stem Cells. (PubMed: 21559406)
- Cancer Res (2011) Human Neural Stem Cell Transplantation Ameliorates Radiation-Induced Cognitive Dysfunction. (PubMed: 21757460)
- PLoS One (2011) Biophysical characteristics reveal neural stem cell differentiation potential. (PubMed: 21980464)
- Stem Cell Res (2011) Computer-Aided 2D and 3D quantification of human stem cell fate from in vitro samples using Volocity high performance image analysis software. (PubMed: 21775237)
- Nature (2011) Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells. (PubMed: 21289626)
- J Neuroimmunol (2010) Cell replacement therapies to promote remyelination in a viral model of demyelination. (PubMed: 20627412)
- Free Radic Biol Med (2010) Consequences of ionizing radiation-induced damage in human neural stem cells. (PubMed: 20826207)