USC Center for Stem Cell and Regenerative Medicine: Shared Research Laboratory and Course in Current Protocols in Human Embryonic Stem Cell Research

USC Center for Stem Cell and Regenerative Medicine: Shared Research Laboratory and Course in Current Protocols in Human Embryonic Stem Cell Research

Funding Type: 
Shared Labs
Grant Number: 
CL1-00524-1.2
Award Value: 
$3,513,204
Stem Cell Use: 
Embryonic Stem Cell
iPS Cell
Cell Line Generation: 
iPS Cell
Status: 
Active
Public Abstract: 
Statement of Benefit to California: 
Progress Report: 

Year 1

The Shared Research Laboratory at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC has provided support and training for stem cell researchers at USC and neighboring institutions. We have supplied training and expertise for a diverse range of research projects on human embryonic stem cells and induced pluripotent stem cells, including one project that evolved into a successful CIRM Disease Team application in the field of macular degeneration. The services supplied by our facility include quality controlled cell lines and reagents, biobanking, and access to communal research space and specialized equipment. The SRL also collaborates with scientists in several research projects. We worked with Dr. Pin Wang to develop new methods for genetic manipulation of human embryonic stem cells. We collaborated with Dr. Gregor Adams in developing techniques for hematopoietic differentiation of human ES cells. Finally the SRL participated in the International Stem Cell Initiative studies of media for propagation of human ES cells and human pluripotent stem cell genetic and epigenetic stability. The SRL is managed by Dr. Victoria Fox with the assistance of four scientific staff. We operate two formal training courses, one on Human Pluripotent Stem Cell Culture, and the second on RNAi Mediated Gene Knockdown in Human Pluripotent Stem Cells. We have had extensive participation in these courses from USC students and staff and from our partners in the CIRM Bridges to Stem Cell Research Program. The SRL has also served as a training facility for interns in the CIRM Bridges to Stem Cell Research Program.

Year 2

The USC Stem Cell Core Shared Research Laboratory (SRL), located at the Eli Edythe Broad CIRM center, is a comprehensive support center and central hub for researchers working with stem cells. This laboratory functions to accelerate research involving pluripotent stem cells through the provision of cells, reagents, equipment, technical assistance, training and shared laboratory space. The SRL has provided a variety of services to over 30 laboratories undertaking a diverse range of projects in the fields of stem cell and developmental biology, regenerative and translational medicine, epigenetics, reprograming, cancer biology, biochemistry and bioengineering. We offer a comprehensive hPSC techniques course and customized ‘modular’ training program to researchers and CIRM bridges students throughout the LA area. Our formal biobanking services enable researchers to bank and preserve hPSC lines without the normal worries associated with cryopreserving these cells. The SRL also operates a research and development program which validates new technologies for maintaining and manipulating human pluripotent stem cells. We are currently centralizing methods for generating human iPSC’s and continue to work on the establishment of improved techniques for genetically modifying hPSC’s. In collaboration with other USC researchers the SRL is working to make transgenic hPSC lines carrying neural reporter genes available to the research community. We are also addressing key issues regarding the in vitro requirements for hPSC during single cell plating.

Year 3

The USC Stem Cell Core Facility is a comprehensive support center for stem cell and biomedical researchers located at The University of Southern California and other neighboring institutions in Los Angeles. It provides research support and training to over 200 individuals ranging from high school students to tenured professors. The users of the facility participate in a wide diversity of basic and translational research involving embryonic and adult stem cells. Some of the services offered by this facility include quality controlled cells and bioreagents, shared equipment and laboratory space, biobanking, a cell repository, histology, karyotyping, technical assistance and consultation. The USC Stem Cell Core staff also participate in research and development aimed to establishing, validating and centralizing new technologies which fuel the production of new services and lower the barriers to stem cell research. The main goal of this facility is to provide support which enables all life science researchers regardless of their expertise to harness the amazing potential of stem cells for studying and treating human diseases.

Year 4

The USC Stem Cell Core Shared Research Laboratory (SRL), located at the Eli Edythe Broad CIRM center, is a comprehensive support center and central hub for researchers working with stem cells. This laboratory functions to accelerate research involving pluripotent stem cells through the provision of cells, reagents, equipment, technical assistance, training and shared laboratory space. This year we added a new iPSC derivation service to our repertoire of research support services. The SRL has provided support to over 30 laboratories undertaking a diverse range of projects in the fields of stem cell and developmental biology, regenerative and translational medicine, epigenetics, reprograming, cancer biology, biochemistry and bioengineering. We offer a comprehensive hPSC techniques course and customized ‘modular’ training program to researchers and CIRM bridges students throughout the LA area. We also run a high school science education and outreach program called the EiHS Summer program in stem cell research, in collaboration with a CIRM funded creativity award. Together these programs enable over 20 students to participate in laboratory research and classroom based forums focusing on stem cell research and regenerative medicine. The SRL also operates a research and development program which validates new technologies for maintaining and manipulating human pluripotent stem cells. We are currently working to centralize methods for genetically modifying hPSC’s. In collaboration with other USC researchers the SRL is working to make transgenic hPSC lines carrying neural reporter genes available to the research community. We are also addressing key issues regarding the in vitro requirements for hPSC during single cell plating.

Year 5

The USC Stem Cell Core Shared Research Laboratory (SRL), located at the Eli Edythe Broad CIRM center at the University of Southern California acts as a hub for dissemination of state-of-the-art technology in hESC research throughout the region. This laboratory functions to accelerate research involving pluripotent stem cells through the provision of quality controlled cells, bioreagents, shared equipment and laboratory space, biobanking, a cell repository, histology, karyotyping, technical assistance and consultation. The facility also provides stem cell culture training for researchers ranging from high school students to tenured professors. This year we expanded our iPSC derivation services and have started collaborating with PI’s to derive iPSC from fibroblasts, utilizing a non-integrating plasmid based technology. The SRL continued to see an increase in the total number of users for equipment usage and stem cell training courses. We participated in collaborative research which has resulted in a publication in the Journal of Biomedical Materials Research. We are continuously aiming to recruit more students for our hPSC techniques courses and host plan to host a techniques course for International researchers in the next reporting period. We further expanded our high school science education and outreach program called the EiHS Summer program in stem cell research, which is run in collaboration with a CIRM funded creativity award program. Together these programs have enabled over 20 high school students to participate in laboratory research and classroom based forums focusing on stem cell research and regenerative medicine last year. In addition to this we continue to work on the establishment of improved techniques for genetically modifying hPSC’s and validation of improved media for maintaining hPSC, particularly following single cell dissociation.

© 2013 California Institute for Regenerative Medicine