A bio-instructive parylene-based conformal coating suppresses thrombosis and intimal hyperplasia of implantable vascular devices.

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Publication Year:
2023
Authors:
Dake Hao, Jonathan Lin, Ruiwu Liu, Christopher Pivetti, Kaeli Yamashiro, Linda M Schutzman, Junichiro Sageshima, Mimmie Kwong, Nataliya Bahatyrevich, Diana L Farmer, Misty D Humphries, Kit S Lam, Alyssa Panitch, Aijun Wang
PubMed ID:
37408799
Funding Grants:
Public Summary:
Implantable vascular devices are widely used in clinical treatments for various vascular diseases. However, current approved clinical implantable vascular devices generally have high failure rates because they do not have a living vessel inside of them. In this study we developed a new vessel graft coated with a novel material designed to generate a new vessel inside of the graft. This coating is designed to catch circulating endothelial cells and prevent platelets from binding to the inner surface of the graft. Also, we confirmed the long-term stability and function of this coating in human serum. Using two vascular disease-related large animal models, a pig carotid artery model and a pig carotid artery-jugular vein arteriovenous graft model, we demonstrated that this coating enabled rapid generation of self-renewable "living" endothelium on the blood contacting surface of the grafts after implantation. We expect this easy-to-apply conformal coating will present a promising avenue to engineer surface properties of "off-the-shelf" implantable vascular devices for long-lasting performance in the operating room.
Scientific Abstract:
Implantable vascular devices are widely used in clinical treatments for various vascular diseases. However, current approved clinical implantable vascular devices generally have high failure rates primarily due to their surface lacking inherent functional endothelium. Here, inspired by the pathological mechanisms of vascular device failure and physiological functions of native endothelium, we developed a new generation of bioactive parylene (poly(p-xylylene))-based conformal coating to address these challenges of the vascular devices. This coating used a polyethylene glycol (PEG) linker to introduce an endothelial progenitor cell (EPC) specific binding ligand LXW7 (cGRGDdvc) onto the vascular devices for preventing platelet adhesion and selectively capturing endogenous EPCs. Also, we confirmed the long-term stability and function of this coating in human serum. Using two vascular disease-related large animal models, a porcine carotid artery interposition model and a porcine carotid artery-jugular vein arteriovenous graft model, we demonstrated that this coating enabled rapid generation of self-renewable "living" endothelium on the blood contacting surface of the expanded polytetrafluoroethylene (ePTFE) grafts after implantation. We expect this easy-to-apply conformal coating will present a promising avenue to engineer surface properties of "off-the-shelf" implantable vascular devices for long-lasting performance in the clinical settings.