CIRM Stem Cell Research Biotechnology Training Program at CSULB
Grant Award Details
Grant Type:
Grant Number:
TB1-01182
Investigator(s):
Award Value:
$3,487,091
Status:
Closed
Progress Reports
Reporting Period:
Year 4
Reporting Period:
Year 5
Reporting Period:
Year 6
Reporting Period:
Year 7
Grant Application Details
Application Title:
CIRM Stem Cell Research Biotechnology Training Program
Public Abstract:
The proposed project has three major goals. The first is educating the public about the medical, biological, and technological advances of stem cell research and recruiting new scientists into the workforce. The second is training the students in the theory and techniques of stem cell research. The third is retaining these trainees in the California workforce by providing specialized training and laboratory internships, which will lead to long-term career opportunities in stem cell research in California. To educate non-scientists and to increase the number of informed California citizens in the theory and potential of stem cell research, a new general education course will be developed at a local community college as a bridge to our comprehensive university program. A new module also will be added to our existing large, lower division, general education lecture course “Introduction to Human Diseases.” This course may be the only life sciences many students will learn in college and could initiate a life-long appreciation of the biological sciences, including stem cell technologies. Such an appreciation will have a significant impact on our society given the role of the voting population in the funding and promoting of advanced technologies. The California stem cell research workforce will be enhanced by recruiting up to ten students each year to enter a new, two-year, stem cell training option which will be added to an existing Biotechnology Certificate Program. The first year will be training at our institution, and the second will be internships at stem cell host institutions Of the approximately 2000 students in Chemistry and the Biological Sciences, those interested in the program will enroll in specific fall semester courses as part of their B.S. or M.S. degree plan, or in the Biotechnology Certificate Program. Exceptional students from this pool who demonstrate reliability and motivation will be invited to apply for the internship. Students who are accepted will attend a stem cell techniques course at [REDACTED] and will choose the host stem cell research laboratory for their ten-month internship at either the [REDACTED] or [REDACTED]. The students will be extensively mentored throughout the program. Trainee progress will be assessed via standardized reporting, which will be completed by the students and the head of the host laboratories. The program will include a program director and an Advisory Committee consisting of the Program Director, two representatives from our institution, and one representative from each of the collaborating institutions. [REDACTED] has a long history of successfully training large numbers of students for the California workforce and for graduate study. The CIRM Bridges to stem cell research training program will integrate well with the existing programs and augment the Biotechnology Certificate Program.
Statement of Benefit to California:
The goals of the proposed program include training students to enter the stem cell research workforce, recruiting students to work in stem cell laboratories, and educating non-scientists in the theory and potential of stem cell research. The proposed project has three major facets. The first is educating the public about the medical, biological, and technological advances of stem cell research and recruiting new scientists into the workforce. The second is training students in the theory and techniques of stem cell research. The third is retaining these trainees in the California workforce by providing specialized training and experience that will lead to career opportunities in stem cell research in California. To educate non-scientists and to increase the number of informed California citizens in the theory and potential of stem cell research, a new general education course will be developed at a local community college as a bridge to the our comprehensive university program. A new module will also be added to our existing large, lower division, general education lecture course “Introduction to Human Diseases”. This course is extremely important because this is, in many cases, the only life sciences many students will learn in college. This course could instill excitement and enthusiasm for life-long learning in students, many of whom dislike or are fearful of science. This is critical for personal well being, and may have a significant impact on our society given the role of the voting population in the funding and promotion of advanced technologies. We enroll just under 38,000 students who reflect the ethnically diverse population of the surrounding area. The stem cell training program can be integrated into many of our existing B.S. and M.S. degree programs, and ensures the currency of courses for many of our students. The Biotechnology Certificate Program was established at our institution in 1994 to train undergraduate, post baccalaureate, and Master’s degree students to enter the California workforce. The stem cell training program will become an option in this program. The current Biotechnology Program is open to all B.S. and M.S. degree students in the departments of Biological Sciences and Chemistry & Biochemistry (approximately 2000 students), and qualified post baccalaureate students. This new option will take two years to complete. The first year will be training at our institution, and the second will be internships at stem cell host institutions. We plan to train up to ten students each year in this internship program. [REDACTED] has a long history of successfully training large numbers of students for the California workforce and for graduate study. Both the students and the State of California will greatly benefit from this training program as it will facilitate the establishment and maintenance of active stem cell research laboratories and the translation of this technology into the regenerative medicine marketplace.
Publications
- Stem Cell Reports (2020): Aberrant Development Corrected in Adult-Onset Huntington’s Disease iPSC-Derived Neuronal Cultures via WNT Signaling Modulation. (PubMed: 32109367)
- Regen Med (2011): Achieving stable human stem cell engraftment and survival in the CNS: is the future of regenerative medicine immunodeficient? (PubMed: 21548741)
- Hum Mol Genet (2023): An altered extracellular matrix-integrin interface contributes to Huntington’s disease-associated CNS dysfunction in glial and vascular cells. (PubMed: 36547263)
- J Neurosci Methods (2018): Astrocyte-enriched feeder layers from cryopreserved cells support differentiation of spontaneously active networks of human iPSC-derived neurons. (PubMed: 28746822)
- Stem Cells Int (2018): Bcl-2 Overexpression Improves Survival and Efficacy of Neural Stem Cell-Mediated Enzyme Prodrug Therapy. (PubMed: 30026762)
- Mol Cancer Ther (2013): Berbamine inhibits the growth of liver cancer cells and cancer-initiating cells by targeting Ca(2)(+)/calmodulin-dependent protein kinase II. (PubMed: 23960096)
- Curr Pathobiol Rep (2013): Bile Acid Receptors and Liver Cancer. (PubMed: 23420103)
- Oncogene (2017): CAMK2gamma antagonizes mTORC1 activation during hepatocarcinogenesis. (PubMed: 27819676)
- Adv Drug Deliv Rev (2017): Cell-mediated enzyme prodrug cancer therapies. (PubMed: 28916493)
- Stem Cell Res (2011): Computer-Aided 2D and 3D quantification of human stem cell fate from in vitro samples using Volocity high performance image analysis software. (PubMed: 21775237)
- Stem Cells Transl Med (2018): Concise Review: Neural Stem Cell-Mediated Targeted Cancer Therapies. (PubMed: 30133188)
- Nat Commun (2023): CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons. (PubMed: 36754966)
- Clin Epigenetics (2017): The effect of Nipped-B-like (Nipbl) haploinsufficiency on genome-wide cohesin binding and target gene expression: modeling Cornelia de Lange syndrome. (PubMed: 28855971)
- Cell Transplant (2017): Effects of Human ES-Derived Neural Stem Cell Transplantation and Kindling in a Rat Model of Traumatic Brain Injury. (PubMed: 28933218)
- Mol Ther Oncolytics (2019): Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer. (PubMed: 30719498)
- Stem Cell Res Ther (2015): Exogenous marker-engineered mesenchymal stem cells detect cancer and metastases in a simple blood assay. (PubMed: 26391980)
- Regen Med (2013): Functional assessment of long-term deficits in rodent models of traumatic brain injury. (PubMed: 23826701)
- PLoS One (2014): GPBAR1/TGR5 mediates bile acid-induced cytokine expression in murine Kupffer cells. (PubMed: 24755711)
- Stem Cells (2012): Human neural stem cell tropism to metastatic breast cancer. (PubMed: 22084033)
- Cell Rep (2017): Huntington’s Disease iPSC-Derived Brain Microvascular Endothelial Cells Reveal WNT-Mediated Angiogenic and Blood-Brain Barrier Deficits. (PubMed: 28514657)
- Proc Natl Acad Sci U S A (2012): Identification of Oct4-activating compounds that enhance reprogramming efficiency. (PubMed: 23213213)
- Cell Stem Cell (2012): Induced Pluripotent Stem Cells from Patients with Huntington’s Disease Show CAG-Repeat-Expansion-Associated Phenotypes. (PubMed: 22748968)
- eNeuro (2021): Interneuron Dysfunction in a New Mouse Model of SCN1A GEFS. (PubMed: 33658306)
- Bioconjug Chem (2017): Intraperitoneal Administration of Neural Stem Cell-Nanoparticle Conjugates Targets Chemotherapy to Ovarian Tumors. (PubMed: 28453256)
- Neuron (2017): iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases. (PubMed: 28426964)
- Nat Commun (2018): KIF20A/MKLP2 regulates the division modes of neural progenitor cells during cortical development. (PubMed: 30006548)
- Methods (2018): Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells – Tools for analyzing dynamics of cell cycle, migration, and lineage selection. (PubMed: 29050826)
- Stem Cells Transl Med (2013): Magnetic Resonance Imaging Tracking of Ferumoxytol-Labeled Human Neural Stem Cells: Studies Leading to Clinical Use. (PubMed: 24014682)
- J Hered (2014): Mapping genes to chicken microchromosome 16 and discovery of olfactory and scavenger receptor genes near the major histocompatibility complex. (PubMed: 24336927)
- Sci Transl Med (2017): Mechanoresponsive stem cells to target cancer metastases through biophysical cues. (PubMed: 28747514)
- Cell Rep Med (2020): Modeling Human Cytomegalovirus-Induced Microcephaly in Human iPSC-Derived Brain Organoids. (PubMed: 33205055)
- Oncogene (2011): NAMPT overexpression in prostate cancer and its contribution to tumor cell survival and stress response. (PubMed: 20956937)
- Stem Cells Transl Med (2013): Neural Stem Cell-Mediated Delivery of Irinotecan-Activating Carboxylesterases to Glioma: Implications for Clinical Use. (PubMed: 24167321)
- Mol Ther Oncolytics (2020): Neural Stem Cells Improve the Delivery of Oncolytic Chimeric Orthopoxvirus in a Metastatic Ovarian Cancer Model. (PubMed: 32775617)
- Graefes Arch Clin Exp Ophthalmol (2014): A new immunodeficient pigmented retinal degenerate rat strain to study transplantation of human cells without immunosuppression. (PubMed: 24817311)
- iScience (2023): Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits. (PubMed: 36590162)
- Proc Natl Acad Sci U S A (2020): Specific targeting of ovarian tumor-associated macrophages by large, anionic nanoparticles. (PubMed: 32732430)
- Development (2018): TCF7L1 suppresses primitive streak gene expression to support human embryonic stem cell pluripotency. (PubMed: 29361574)
- Cell Stem Cell (2013): Tet1 regulates adult hippocampal neurogenesis and cognition. (PubMed: 23770080)
- Nat Commun (2016): The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. (PubMed: 26965827)
- Stem Cells (2011): Transcriptome Analysis of Neural Progenitor Cells by a Genetic Dual Reporter Strategy. (PubMed: 21805534)
- Exp Neurol (2016): Transplantation of human neural stem cells restores cognition in an immunodeficient rodent model of traumatic brain injury. (PubMed: 27079998)
- Hepatology (2016): Vertical sleeve gastrectomy activates GPBAR-1/TGR5 to sustain weight loss, improve fatty liver, and remit insulin resistance in mice. (PubMed: 27312543)
- Leukemia (2013): The viral oncogene Np9 acts as a critical molecular switch for co-activating beta-catenin, ERK, Akt and Notch1 and promoting the growth of human leukemia stem/progenitor cells. (PubMed: 23307033)