Stage of Program: Basic Research and Discovery Stage Programs
Endodermal differentiation of human ES cells
The goals of this proposal are to investigate endodermal differentiation and proliferation in human ES cell cultures. Endodermal cells give rise to the epithelial lining of the respiratory and digestive tract as well as to the liver and pancreas. The future treatment of diseases such as type I diabetes using stem cell therapy relies on […]
A Chemical Approach to Stem Cell Biology
The aim of this project is to screen large collections of small molecules to identify molecules that allow one to propagate human embryonic stem cells (hESCs) in cell culture under defined conditions in an undifferentiated, pluripotent state. The chemical structures of any biologically active small molecules will be optimized with respect to potency, selectivity and […]
Functions of RB family proteins in human embryonic stem cells
Nearly one out of every two Californians born today will develop cancer at some point in their lives, and it is likely that one in five persons will die of the disease. We propose to study the mechanisms of action of the RB gene, which is mutated in a broad range of human cancers, including […]
Mapping the transcriptional regulatory elements in the genome of hESC
The human embryonic stem cells (hESC) have the remarkable potential to replicate themselves indefinitely and differentiate into virtually any cell type under appropriate environmental conditions. They accomplish this through regulating the production of a unique set of proteins in the cells, a process known as gene regulation. While the genes encoding these stem cell proteins […]
Stem Cell Survival and Differentiation Through Chemical Genetics
This project will test the effects of chemical compounds similar to conventional pills for their abilities to keep human embryonic stem cells growing and multiplying in the laboratory or to help them become one of the specialized types of cells, like spinal cord cells, found in the human body. Many of the substances currently used […]
Regulation of human neural progenitor cell proliferation by Ryk-mediated Wnt signaling
Human ES cells can be used to make healthy neurons to replace the cells that are lost in neurological diseases such as Alzheimer’s and Parkinson’s diseases. This holds great hope for future treatment of these disorders. Our research aims to produce neurons from ES cells more efficiently. During the process of differentiation to form nerve […]
Generation of long-term cultures of human hematopoietic multipotent progenitors from embryonic stem cells
For many therapeutic reasons it is important to have available large numbers of blood cells. However, it is difficult to generate large numbers of specialized blood cells that have the ability to neutralize autoimmunity and response to tumor cell growth. In this study we would develop a technique that would allow the production of large […]
hESC as tools to investigate the neural crest origin of Ewing’s sarcoma
Human embryonic stem cells (hESC) hold great promise as sources of tissue for regenerative medicine and therapeutics. In addition, their utility as tools to study the origins and biology of human disease must not be underestimated. hESC give rise to tissue-specific adult stem cells and, ultimately, to all mature tissues in the body. As such, […]
Differentiation of Human Embryonic Stem Cells to Intestinal Fates
The roughly 25 feet of intestine in the adult human play numerous essential roles in daily life, such as nutrient absorption, secretion of hormones, and serving as a barrier to infection. Commensurate with these diverse roles, diseases of the intestine are a considerable source of human morbidity and mortality. Indeed, numerous pathologic conditions including inflammatory […]
Micro Platform for Controlled Cardiac Myocyte Differentiation
Congestive heart failure, the inability of the heart to continue to pump effectively due to damage of its muscle cells, affects approximately 4.8 million Americans and is a leading cause of mortality. Causes of the irreversible damage to the cardiomyocytes that results in congestive heart failure include hypertension, heart attacks, and coronary disease. Because the […]