Year 2

We have developed a new technique for measuring and correcting the wavefront aberrations introduced by a biological sample (drosophila embryo) using a Shack–Hartmann wavefront sensor, a fluorescent reference source (a bead or a fluorescent protein), and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging.The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved.