Generating pluripotent cell lines from neurons.
Generating pluripotent cell lines from neurons.
New Faculty I
Stem cell research holds great promise for neurological disease. One in three Americans will suffer from diseases of the nervous system ranging from stroke to Alzheimer’s disease to epilepsy. Very few treatments for neurological disease exist, in part because of he lack of suitable in vitro models with which to test therapeutics. In addition, many neuronal disorders, including Parkinson’s disease and ALS, are characterized by loss of important subpopulations of neurons. In affected patients, the only way to restore function may be to provide them with replacement neurons. Many researchers are already working on methods to generate replacement neurons from human embryonic stem cells or to generate accurate in vitro models of neurological diseases. Here, we propose to perform the reverse experiment; we aim to generate pluripotent cell lines directly from neurons, using two novel technologies. The first goal of these experiments is to generate cell lines so that we can compare the chromosomes of neurons with those of neurons derived from ES cells. If differences exist, and are important for the proper function of neurons, it is essential to identify these changes. Similarly, if neurons in diseased patients have DNAchanges that cause disease symptoms, it would be better to derive ES cells directly from neurons and then to “re-differentiate ” them into better in vitro models for drug screening. Both of these findings will significantly impact the ~ 30% of CIRM funded grants aimed at curing various diseases of the nervous system.
Statement of Benefit to California:
The goal of this study is to develop novel techniques to generate stem cell lines directly from neurons, which is currently impossible in humans. Our findings will also allow us to validate or improve current strategies to generate replacement neurons from human embryonic stem cells. Our experiments should suggest new ways to derive patient specific cell lines to treat or study common human neurological diseases such as Alzheimer’s and autism. These findings may lead to relief for patients who suffer from currently untreatable diseases of the nervous system. In addition, our novel methods may foster innovation in the dynamic biotechnology and health-care sectors of the California economy, which would benefit many Californians in by creating jobs and promoting economic growth.
Year 1The goal of our research is to generate cell lines from neurons with the long term goal to understand how to generate the best possible stem-cell derived models for human neurological diseases such as Alzheimer's, Parkinson's and Huntington's diseases. These studies might also reveal basic mechanisms of disease that could inspire new drug development efforts. We proposed to use two cutting edge methods in stem cell biology to accomplish this: cloning and direct reprogramming (generation of induced pluripotent stem cells with reprogramming factors). At the time, methods for direct reprogramming were in their infancy and it was not possible to generate mice entirely from these cells. Because reprogramming neurons is likely to be inefficient and difficult, we proposed to generate entire mice derived from reprogrammed cells. Al cell types, including neurons in these mice would carry special genes to allow us to then turn their neurons into stem cell lines. We have successfully generate such mice and in doing so, we became the first to show that fertile adult mice could be generated from skin cells using induced pluripotent stem cells. This result helps to validate the utility of stem cells and induced pluripotent stem cells and represents the achievement of a critical milestone in our project timeline. Present efforts will use these mice as a unique resource to achieve the goal of the application.
Year 2The goal of this grant is to generate cell lines from mouse neurons in order to learn how to produce the best possible models of neurological disease. Neurons do not divide and neurons from adults cannot be grown in a dish so very little is known about the genomes of neurons. We have developed reprogramming methods to generate cell lines from neurons and we have established methods to perform whole genome sequencing of pluripotent cell lines that establish whether mutations derive from donor cells or arose during reprogramming. By combining these methods we will be able to survey the genomes of neurons and determine whether it is important to use neurons as sources to generate cell lines for disease modeling.
Year 3The goal of this grant is to generate stem cell lines from neurons. This is important because neurons are affected in many diseases yet they cannot be made into dividing cell lines using standard methods and therefore it is difficult to study human neurologic disease. A second goal is to use neuron-derived cell lines to determine whether neurons harbor genomic mutations or stable DNA marks that are relevant to brain development and/or human neurologic disease. We have successfully generated the first mouse pluripotent cell lines that are derived from adult cortical neurons and are in the process of using whole genome sequencing to identify any stable DNA marks that are unique to neurons. Results of this research will help to establish the best methods for modeling human neurological disease using stem cell technologies and establish the extent and identity of stable DNA changes in cortical neurons.
- Cell Stem Cell (2011) Genome sequencing of mouse induced pluripotent stem cells reveals retroelement stability and infrequent DNA rearrangement during reprogramming. (PubMed: 21982236)
- Nature (2011) Sensory maps in the olfactory cortex defined by long-range viral tracing of single neurons. (PubMed: 21451523)
- Nature (2009) Adult mice generated from induced pluripotent stem cells. (PubMed: 19672243)