In Vitro Differentiation of T cells from Human Embryonic Stem Cells.

In Vitro Differentiation of T cells from Human Embryonic Stem Cells.

Funding Type: 
SEED Grant
Grant Number: 
RS1-00295
Award Value: 
$477,894
Stem Cell Use: 
Embryonic Stem Cell
Status: 
Closed
Public Abstract: 
Statement of Benefit to California: 
Progress Report: 

Year 1

T cells orchestrate immune responses and the ability to generate T cells from embryonic stem cells in a test tube could provide potentially limitless source of customized T cells for a variety of conditions including: cancer, infection and autoimmune diseases. In the past year of CIRM funding we have made progress toward this goal by identifying individual embryonic stem cell lines that have a tendency to give rise to blood cell types, including T cells. We have investigated improvements to the culture system, including manipulating extracellular signals.

Year 2

T cells orchestrate immune responses and the ability to generate T cells from embryonic stem cells in a test tube could provide potentially limitless source of customized T cells for a variety of conditions including: cancer, infection and autoimmune diseases. In the past year of CIRM funding we have made progress toward this goal by comparing different culture conditions and distinct cell lines for their ability to generate blood cell types, including T cells in vitro. In the future, we plan to combine studies in humanized mouse models with in vitro approaches to overcome the bottleneck generating T cells from stem cells.

© 2013 California Institute for Regenerative Medicine