Year 1

The overall goal of Year 1 project is to obtain preliminary data that is essential to develop a large scale bioreactor for a clinical extracorporeal liver support system. The international multidisciplinary teams aimed and completed each specific aim; (1) optimizing co-culture system with human iPS-derived hepatic cells and non-parenchymal cells (LX2 and HUVEC) in the conventional 2D culture, (2) optimizing bioreactor design to suitable for human iPS cell culture and hepatic differentiation, and (3) establishing undifferentiated human iPS cells expansion in the 3D bioreactor.