Year 2

During the past year of support, we generated the knock-in hESCs to introduce a florescent marker into the human hematopoietic stem cell (HSC)-specific locus. Together with CD34 marker, we were able to purify and enrich hESC-derived HSCs. We differentiate hESCs into cardiomyocytes and showed that the humanized mice with functional human immune system that can reject the cardiomyocytes from another individual.